Demystifying rennet, a key ingredient in cheesemaking (2024)

We all know the main ingredient in cheese is milk (and we spend a lot of our time talking about it), but what else is in cheese? Most cheeses are comprised of milk, salt, cultures and rennet. Rennet is the most fascinating of these ingredients, yet it is a mystery to many.

Although the origins of cheesemaking are murky and still much debated, one of my favorite “origin tales” is that of the nomadic shepherd who was traveling with milk in a sack made from the stomach of a young animal. Upon arriving at his destination, the shepherd went to take a swig of milk only to find that it had curdled into a delicious solid. While this is probably just a fable, it still speaks to a huge discovery in the science of cheesemaking! Something magical happens to milk in the stomachs of young ruminants that helps cheesemakers achieve our primary objective: to extend milk’s “shelf life”. Rennet is the magic!

But first, a little science primer. Milk is an emulsion of fat, protein, sugar and minerals suspended in water. Cheesemakers use one of a few different methods to trigger a chemical reaction that causes coagulation, transforming liquid milk into a solid coagulum, or curd. Coagulation occurs when proteins clump together, trapping fat and other solids like a sponge. For cheddar, a hard aged cheese, we use enzymatic coagulation--by adding rennet. For more on other methods of coagulation, check out this great article on Cheese Science Toolkit

Phew! Now that some of the chemistry is out of the way, what exactly is rennet? And where did it come from? Rennet is the general name for enzymes that act on proteins in milk. It’s purpose in a ruminant’s stomach is to curdle milk for easier digestion, the same way it curdled our shepherd’s drink. Rennet serves the same purpose in cheesemaking: it triggers coagulation.

Demystifying rennet, a key ingredient in cheesemaking (2)

Demystifying rennet, a key ingredient in cheesemaking (3)

Demystifying rennet, a key ingredient in cheesemaking (4)

There are several types of rennet. At Shelburne Farms, we utilize traditional calf rennet. Calf rennet contains two enzymes, chymosin and pepsin, in a natural ratio that is suitable for our style of cheese. (Chymosin causes coagulation, pepsin has impacts on the aging process.) This traditional rennet has more reliable results in yield, as well as flavor profile, leading to less defective cheese. It is also a natural and non-GMO option that exemplifies our efforts to be sustainable by utilizing all parts of the animal.

In some cultures, vegetable and plant rennet has been used to coagulate milk. In India, where the cow is revered for religious reasons, fruit trees were used. Many Spanish sheeps’ milk cheeses use thistle and cardoon rennets. One drawback of these coagulants is they may lead to bitter and undesirable flavors in cows’ milk cheeses.

A demand for an inexpensive and reliable alternative to calf rennet led to the development of a genetically modified bacterium that can produce rennet enzymes through fermentation, calledfermentation produced chymosin. In 1990, Pfizer successfully produced a chymosin that is genetically identical to the chymosin found in calf rennet. It is filtered from the bacteria in which it’s grown and isolated. Fermentation produced chymosin does not contain pepsin, so for our purposes, it does not have the exact same impact on the aging process.

Calf rennet is now the only rennet we use here as it is best for the flavor profile we are seeking. We haveused fermentation produced chymosin at Shelburne Farms in the past, and we believe the product is safe and reliable, and can be part of a healthy food system. Debate on whether these enzymes are effectively a genetically modified organism is ongoing. As of 2008, according to the culture houses who produce these products, 80-90 percent of cheeses in the United States and Britain utilize fermentation-produced chymosin. At the same time, it has been banned in several countries that do not allow genetic engineering of food products.

I hope this introduction to rennet helps to demystify this important cheese ingredient. As with all our food choices, understanding how a product is made can help us make more informed decisions. Still, the world of rennet is particularly complicated, both scientifically and chemically, and it raises important questions around food system health. For small cheese makers in Vermont, the choice of which rennet to use is not a simple one.

If you want to dive even deeper into this topic, check out the entry on “rennet” in the Oxford Companion to Cheese, edited by UVM professor Catherine Donnelly, with a forward by Jasper Hill Farm founder Mateo Kehler. UVM professor Paul Kindstedt has written two definitive books on cheese that cover rennet at length, American Farmstead Cheese and Cheese and Culture. (You can find copies at our Welcome Center and Farm Store.)

Demystifying rennet, a key ingredient in cheesemaking (2024)

FAQs

Demystifying rennet, a key ingredient in cheesemaking? ›

Rennet is the general name for enzymes that act on proteins in milk. It's purpose in a ruminant's stomach is to curdle milk for easier digestion, the same way it curdled our shepherd's drink. Rennet serves the same purpose in cheesemaking: it triggers coagulation.

What happens if you use too much rennet in cheese making? ›

Too much rennet was used or too much butterfat, left your cheese during the process. Rubbery cheese can happen when an excess amount of rennet is used, too much rennet equals a rubber ball, too little, soup!

What is rennet and why is it important in cheese making? ›

Rennet isolates cheese curds from liquid whey, and it causes those curds to clump together. It does this by targeting casein, the primary protein in milk. Enzymes affect the behavior of proteins, and in the case of rennet, it causes casein molecules to divide and re-coagulate into even larger clumps.

What is the best rennet for cheese making? ›

Different Types of Rennet

However, tablet and powdered rennet can be stored for longer periods of time and survive better in warmer climates. Calf rennet is considered to be the best choice for longer aged cheese, because some of its residual components help to complete the breakdown of proteins.

What are the four 4 basic ingredients for cheese production? ›

Natural cheese is made from four basic ingredients including milk, salt, a “good bacteria” and rennet, an enzyme. From there, cheesemakers can adjust the basic recipe by adding other ingredients to make all of the cheeses we know and love.

How much rennet for 1 gallon of milk? ›

The recipes in our kits typically call for 1/4 tablet for 1 gallon of milk. Ten tablets will set a minimum of 40 gallons of milk. Crush desired portion of tablet and dissolve in 1/4 cup of cool, non-chlorinated water. Add to milk when specified in recipe.

Is rennet or vinegar better for making cheese? ›

The lemon juice, vinegar and citric acid can coagulate the milk without rennet but frequently work best with hot milk. The use of rennet allows to separate the milk before the acidification of the milk is too high and helps form hard curds and can be used at moderate milk temperature for an extended time.

Are calves killed for rennet? ›

Parmesan cheese is made with animal rennet which is an enzyme that comes from the fourth lining of the stomach of animals, typically calves, goats, and lambs. The animal is usually killed in the process. The rennet is then taken and dried so it becomes solid.

Can cheese be made without rennet? ›

Younger, soft cheeses like mozzarella, cottage cheese, cream cheese, ricotta, goat, and provolone often are not made with rennet. However, this isn't an exhaustive list, so be sure to read your labels carefully!.

Does tillamook cheese use Pfizer rennet? ›

The exact method of making this fermentation-produced rennet is proprietary. However, it is important to note that our rennet is classified as “not genetically engineered” by USDA bioengineered food labeling regulations. Furthermore, we do not use rennet that is produced by any pharmaceutical company.

Can I use expired rennet? ›

After a few months past the best before date has been reached it is recommended that they are discarded, and a new batch used. Rennet is such a key ingredient in making cheese that my recommendation is to make cheese using a rennet where the best before date has not expired.

Can I make my own rennet? ›

Instructions for Making Nettle Rennet
  1. Rinse 2 pounds fresh leaves under cool, filtered water.
  2. Fill a large pot with 4 cups water. ...
  3. Add 1 heaping tablespoon of sea salt to the pot; stir gently to dissolve. ...
  4. Place a colander inside a large bowl. ...
  5. The liquid drained from the nettle leaves is the liquid nettle rennet.

What are the 4 types of rennet? ›

There are four main types of rennet that cheesemakers can choose from- animal rennet, microbial rennet, plant rennet, and fermentation-based rennet. The two most popular types of rennet are microbial and animal rennet. Without rennet, there would be no coagulation; meaning milk would never curd into cheese.

Is rennet still used to make cheese? ›

Historically, rennet was harvested from the stomach lining of young cows, sheep, and goats, and many cheeses are still made with animal rennet.

What is a person who makes cheese called? ›

What do you call someone who makes cheese? Simply: a cheesemaker. Or if you're trying to impress someone, you could go with the French word, fromager.

What are the 3 ingredients used to make the majority of cheese? ›

Most types of cheese only need two or three ingredients, milk, cultures and rennet. These simple ingredients will ripen the milk, form curds and whey and add flavor to the finished cheese.

How much rennet is used to make cheese? ›

Also, different types of cheese need different setting times. Some of my recipes state that you use 2.5 ml (1/2 teaspoon) of single strength rennet to set 10 litres and others state to use 5 ml (1 teaspoon) to set the same amount.

Can you add more rennet to cheese? ›

CURDS ARE TOO SOFT

If it does not improve, this problem may come from ultra-pasteurization or ultra-hom*ogenization of the milk. You can try adding more rennet, depending on where you are in the cheesemaking process. You should add more rennet only to milk that has not yet been cut into curds.

Why is my homemade cheese not melting? ›

Why won't my cheese melt? There are many reasons, but the biggest is the moisture content. The moisture content of the cheese has a great effect on the melting process. Cheese that contains higher amounts of fat will melt better than one that doesn't.

Why is my homemade mozzarella not stretchy? ›

For Mozzarella to stretch well it needs two things: The right amount of heat to soften the curd. The right acidity (pH of approximately 5.2)

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